Strict global regulatory limits govern how much aflatoxin M1 may be present in dairy products. Using monoclonal antibody-based affinity chromatography, Afla M1 FL+ is a quantitative method for the fluorometer based detection of aflatoxin M1 in milk at parts per trillion (ppt) levels. Afla M1 FL+ can be performed rapidly, requires no special skills and is useful for both the milk processing QC and government testing laboratory.